There are three classes of BSCs: Class I, II and III. While all three classes afford personnel and environmental protection, only Class II and III cabinets provide product protection thanks to the input of clean air from supply HEPA filters.
Class I BSCs are suitable for work involving low- to moderate-risk agents. Since incoming air is not filtered, Class I BSCs should not be used with research materials (e.g., cell lines) that must be handled under sterile conditions. Class I cabinets are used specifically to enclose equipment (e.g., centrifuges, harvesting equipment or small fermenters) or procedures with potential to generate aerosols (e.g., cage dumping, tissue homogenization or culture aeration). As outgoing air is filtered, research personnel is protected while using a Class I BSC.
Class II BSCs provide an effective partial barrier system for the safe manipulation of low-, moderate-, and high-risk microorganisms. Class II cabinets, which are the most frequently used in research and clinical laboratories, are divided into four types (Types A1, A2, B1 and B2).
The Class III BSC is a totally enclosed, gas-tight ventilated cabinet, and provides the highest level of personnel, environmental and product protection. Operations within a Class III BSC are conducted through attached rubber gloves.
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